One of the major difficulty in stem cell linical application is the inability of cellular structures to integrate and survive and repair the damaged target tissues. Injected single cells also survive poorly and can hardly integrate in tissues.
A practical solution is to deliver the cells on small particulate matrix, in a manner that can allow their integration in the tissues. Fibrin micro-beads has higher cell binding capability than native fibrin gel. FMB bind matrix dependent cells and this may be used for the isolation of mesenchymal stem cells.
It is thought to have higher yield than conventional methods and expand them in suspension culture without the need of passages. Cell on fibrin micro bead are claimed to differentiate into different phenotypes, such as bone and cartilage, better cell survival and integration in the target organs.
What Is The Need For Alternative?
Regenrative Medicine wants to address the challenge of regenrating the complex organs. One of the approches were proposed by methods of tissue engineering. ytis involved constructing in vitros with potent stem cell. The technique involved-
- Building in-vitro cellular scaffolds from synthetic or biological polymeric materials
- Integrating either isolated differentiated cells of the damaged tissue or pluri-potent stem cells with the addition of cocktails of growth factors
The expectationthat successful implantation can be achieved only by incorporating a scaffold of choice, optimal cells type, preferably stem cells, and growth factors is not realistic. I
One of the solutions for cell delivery is to attach the cells on particulate cell binding matrices that carry the cells and maintain vitality from adjacent circulation.
It is claimed micro-carrier scaffolds that may enable the implant and integration of the cells in the damaged tissues are practical solution.
Only the time would tell.
About Arun Pal Singh